RapiClear 1.52

▪ 不同于脂溶性透明剂，此透明剂为水溶性透明剂，使用方便、透明可逆。

▪样品可以直接从水，缓冲溶液和甘油转移到RapiClear 1.52透明剂中。

▪ 如果样品重新浸入水或缓冲溶液中，透明效果是可逆的。

▪ RapiClear 1.52即用型，无需离心。

▪ RapiClear 1.52使得在组织表面下方1~5 mm处目标清晰可见。

▪ RapiClear 1.52的应用仅引入了有限的样品变形。

▪ RapiClear 1.52是一种抗冻液体。 RapiClear 封片后，样品可在-20℃下长期保存。

▪ RapiClear 1.52的折射率nD = 1.52，接近盖玻片和油镜油的折射率。

 

---

应用：

▪ 动物组织切片> 0.5mm厚度

▪ 鼠器官

▪ 肿瘤

相关产品：

▪ iSpacer imaging Spacer/成像垫片（货号# IS001）

▪ RapiClear CS mounting solution（货号# RCCS001）

▪ RapiClear CS mounting gel（货号# RCCS004）

 

样品				适用产品
动物	昆虫	果蝇、蝗虫、蟑螂等的组织		RapiClear 1.47
	小鼠	组织切片 (厚度)	<0.5 mm	RapiClear 1.47
			>0.5 mm	RapiClear 1.49
				RapiClear 1.52
		器官	脑、肾脏和心脏	RapiClear 1.55
			胃，肠，肝，肺，胰腺，皮肤等	RapiClear 1.52
	大鼠	组织切片 (厚度)	<0.5 mm	RapiClear 1.47
			>0.5 mm	RapiClear 1.49
				RapiClear 1.52
	斑马鱼	幼鱼		RapiClear 1.47
		成年鱼		RapiClear 1.49
生物材料	Matrigel, collagen matrix, agarose,			RapiClear 1.47
植物	拟南芥（A. thaliana）、水稻、烟草等的组织			RapiClear 1.47

相关文献:

1.  Patel, J., Wong, H.Y., Wang, W., Alexis, J., Shafiee, A., Stevenson, A.J., Gabrielli, B., Fisk, N.M., Khosrotehrani, K.* (2016). Self-Renewal and High Proliferative Colony Forming Capacity of Late-Outgrowth Endothelial Progenitors is Regulated by Cyclin-Dependent Kinase Inhibitors Driven by Notch Signaling. Stem Cells. doi: http://dx.doi.org/10.1002/stem.2262 *corresponding authors
2. Lin, P.Y., Peng, S.J., Shen, C.N.*, Pasricha, P.J.*, Tang, S.C.* (2016). PanIN-associated pericyte, glial, and islet remodeling in mice revealed by 3-D pancreatic duct lesion histology. Am J Physiol Gastrointest Liver Physiol. doi: http://dx.doi.org/10.1152/ajpgi.00071.2016 *corresponding authors
3. Chien, H.J.#, Peng, S.J.#, Hua, T.E.#, Kuo, C.H., Juang, J.H., Tang, S.C.* (2016). 3-D imaging of islets in obesity: formation of the islet-duct complex and neurovascular remodeling in young hyperphagic mice. Int J Obes. doi: http://dx.doi.org/10.1038/ijo.2015.224 *corresponding author, #equal contribution
4.  Chappell, J., Harman, J.L., Narasimhan, V.M., Yu, H., Foote, K., Simons, B.D., Bennett, M.R., Jorgensen, H.F.* (2016). Extensive Proliferation of a Subset of Differentiated, Yet Plastic, Medial Vascular Smooth Muscle Cells Contribute to Neointimal Formation in Mouse Injury and Atherosclerosis Models. Circ Res. doi: http://dx.doi.org/10.1161/CIRCRESAHA.116.309799 *corresponding author
5. Leushacke, M., Tan, S.H., Wong, A., Swathi, Y., Hajamohideen, A., Tan, L.T., Goh, J., Wong, E., Denil, S.L.I.J., Murakami, K., Barker, N.* (2017). Lgr5-expressing chief cells drive epithelial regeneration and cancer in the oxyntic stomach. Nat Cell Biol. doi: http://dx.doi.org/10.1038/ncb3541 *corresponding author
6. Tang, S.C. #*, Shen, C.N. #*, Lin, P.Y., Peng, S.J., Chien, H.J., Chou, Y.H., Chamberlain, C.E., Pasricha, P.J. (2017). Pancreatic neuro-insular network in young mice revealed by 3D panoramic histology. Diabetologia. doi: http://dx.doi.org/10.1007/s00125-017-4408-y *corresponding authors, #equal contribution
7. Rulands, S., Lescroart, F., Chabab, S., Hindley, C.J., Prior, N., Sznurkowska, M.K., Huch, M., Philpott, A., Blanpain, C., Simons, B. D.* (2018). Universality of clone dynamics during tissue development. Nature Physics. doi: http://dx.doi.org/10.1038/s41567-018-0055-6 *corresponding author 
8. Edwige Roy, Ho Yi Wong, Rehan Villani, Thomas Rouille, Basit Salik, Seen Ling Sim, Valentine Murigneux, Mitchell S. Stark, J. Lynn Fink, H. Peter Soyer, Graeme Walker, J. Guy Lyons, Nicholas Saunders, Kiarash Khosrotehrani, Regional Variation in Epidermal Susceptibility to UV-Induced Carcinogenesis Reflects Proliferative Activity of Epidermal Progenitors, Cell Reports, Volume 31, Issue 9, 2020, 107702, ISSN 2211-1247, https://doi.org/10.1016/j.celrep.2020.107702.
9. Tang, S.C. #*, Baeyens, L.#, Baeyens, L., Peng, S.J., Chien, H.J., Scheel, D.W., Chamberlain, C.E., German, M.S.# (2017). Human pancreatic neuro-insular network in health and fatty infiltration. Diabetologia. doi: http://dx.doi.org/10.1007/s00125-017-4409-x *corresponding author, #equal contribution