Specifications for CD45RA MicroBeads, human

 

Overview

CD45RA MicroBeads were developed for positive selection or depletion of lymphocyte subsets from human PBMCs.

 

Detailed product information

Background information

CD45RA is expressed on naive CD4+ and CD8+ T cells as well as on CD8+ effector T cells. CD45RA is also present on subsets of B and NK cells as well as on plasmacytoid dendritic cells. The CD45RA antibody recognizes the 220 kDa isoform of the leukocyte common antigen (LCA).

CD45RA MicroBeads are used for the positive selection or depletion of CD45RA+ cells. For isolation of CD45RA+ T cell subsets, the CD45RA MicroBeads can be combined with MACS® Cell Isolation Kits6,7 or CD4 and CD8 MultiSort Kits1,2. The CD45RA MicroBeads can also be used to isolate CD45RO+ cells from pre-sorted CD4+ or CD8+ T cells by depletion of CD45RA+ cells.3,4,5,7

 

Downstream applications

Cells isolated with CD45RA MicroBeads can be used for various studies, such as cytokine expression or receptor signaling.

 

Columns

For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.