数据

← iPSC免疫荧光染色（TRA-1-60，SSEA4，NANOG，OCT4，DAPI）

↓注射免疫缺陷小鼠8周畸胎瘤(左 1)和三胚层 H.E.染色结果(左2，右1，右2 )

注意：人胚胎干细胞（hES）或诱导多能干细胞(hiPS)在无滋养层细胞培养条件下培养换液时，请将新鲜的培养液沿培养容器壁缓慢添加，避免液体的冲击力将贴壁的细胞吹起脱离培养皿。

参考文献

1.Yu Wang, Lisha An, Huimin Gao, Chuan Zhang, Lin Wang, Yanping Zhang, Lu Zhang, Yousheng Yan, Xiaohua Jin, Xu Ma, (2025) Generation of a human induced pluripotent stem cell line (NRIFPi001-A) derived from a patient with phenylketonuria (PKU) harboring compound heterozygous variant (c.1199 + 502A＞T and c.728G＞A) in PAH gene, Stem Cell Research, Volume 87, 2025, 103778, ISSN 1873-5061, https://doi.org/10.1016/j.scr.2025.103778.

2. Zhang Haiyana,Ma Yanyana,Yu Shujuanb,et al. Establishment of a human iPSC line (SDQLCHi010-A) from a patient with optic nerve malformation carrying a heterozygous mutation in PAX6 gene.Stem Cell Research.Stem Cell Research 41 (2019) 101611

3.Wen W, Zhang JP, Chen W, Arakaki C, Li XL, Baylink D, Botimer GD, Xu J, Yuan W, Cheng T*, Zhang XB*. Generation of integration-free induced pluripotent stem cells from human peripheral blood mononuclear cells using episomal vectors. J Vis Exp. 2017 Jan 1;(119). doi: 10.3791/55091.

4. Wen W#, Zhang JP#, Xu J#, Su RJ, Neises A, Ji GZ, Yuan W, Cheng T*, Zhang XB*. Enhanced generation of integration-free iPSCs from human adult peripheral blood mononuclear cells with an optimal combination of episomal vectors. Stem Cell Reports. 2016 Jun 14;6(6):873-84.6.

5.Su RJ, Neises A, Zhang XB: Generation of iPS cells from human peripheral blood mononuclear cells using episomal vectors. Methods Mol Biol. 2016;1357:57-69.5.

6.Su RJ, Baylink DJ, Neises A, Kiroyan JB, Meng X, Payne KJ, Tschudy-Seney B, Duan Y, Appleby N, Kearns-Jonker M, Gridley DS, Wang J, Lau KHW, Zhang XB: Efficient generation of integration-free iPS cells from human adult peripheral blood using BCL-XL together with Yamanaka factors. PLoS One. 2013, 8(5): e64496.3.

7.Zhang XB: Cellular reprogramming of human peripheral blood cells. Genomics Proteomics Bioinformatics. 2013 Oct;11(5):264-74.4.

8. Meng X, Neises A, Su RJ, Payne KJ, Ritter L, Gridley DS, Wang J, Sheng M, Lau KH, Baylink DJ, Zhang XB: Efficient reprogramming of human cord blood CD34+ cells into induced pluripotent stem cells with OCT4 and SOX2 alone. Molecular Therapy, 2012;20(2):408-16.2.