检测灵敏度	50 pg/mL
测定范围	0.05-40 ng/mL
检测样本类型/体积	{Serum=50 µL, Plasma=50 µL, Supernatant=100 µL}
手工操作时间	1 hr 20 min
总时间	4 hr 45 min
均相（无需洗涤）	No
批间差异	<12%
批内差异	<10%
仪器设备	Absorbance Microplate Reader
产品规格	96 tests
产品成分	Pre-coated 96 well plate Standard Assay Diluent concentrate Biotinylated Detection Antibody SAV-HRP Wash Buffer Chromogen Stop Solution Adhesive Plate Covers
运输条件	Wet ice
储存温度	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称	Human IgG1
蛋白家族	Other Proteins
物种	Human
检测试剂盒格式	Sandwich ELISA Kit
检测抗体结合物	Biotin
标签或染料	HRP

 

The Human Immunoglobulin G1 (Hu IgG1) ELISA quantitates Hu IgG1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IgG1.

 

原理方法  

 

The Human IgG1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

 

严格验证  

 

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

 

靶标信息  

 

Human IgG1, also known as human immunoglobulin G1, is a subclass of human IgG antibodies that is produced by the immune system in response to antigens. It is the most abundant subclass of IgG in the blood and plays a key role in defense against pathogens. IgG1 works by binding to specific antigens and promoting their clearance from the body through various mechanisms, including phagocytosis and complement activation. IgG1 is also involved in other immune responses, such as antibody-dependent cellular cytotoxicity and neutralization of toxins. Human IgG1 can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious and autoimmune diseases.