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Human LAP/TGFb ProQuantum Immunoassay Kit
- 类型：ProQuantum
- 品牌：Thermo Fisher
- 种属：Human应用：ELISA
规格: 96Tests
货号: A45730
价格: ¥6961.00
优惠: ¥4525.00
规格: 5×96Tests
货号: A457305
价格: ¥31326.00
优惠: ¥20362.00
| 检测灵敏度 | <15 pg/mL |
| 检测范围 | 16-250,000 pg/mL |
| 样本类型/体积 | {Plasma=2-5 µL, Serum=2-5 µL, Supernatant=2-5 µL} |
| 手工操作时间 | 30 min |
| 总时间 | 2 hr |
| 均相（无需洗涤） | Yes |
| 检测仪器 | qPCR Instruments |
| 产品规格 | 96 Tests |
| 产品成分 |
Assay Dilution Buffer Antibody-Conjugate A Antibody-Conjugate B Antibody-Conjugate Dilution Buffer Standard Master Mix Ligase |
| 运输条件 | Dry ice |
| 储存温度 | -20°C |
| 蛋白名称 | LAP |
| 物种（Tested） |
Human |
| 试剂盒类型 | Proximity-based Immunoassay |
| 标签或染料 | FAM™ |
The ProQuantum Human LAP/TGFb Immunoassay Kit is designed to provide quantitative measurements of human LAP/TGFb in small sample volumes. Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive software for analytics enables sample-to-answer in just 2 hours.
• High sensitivity-detect low levels of protein with greater sensitivity than traditional methods
• Broad dynamic range-5 logarithmic units, minimizing sample dilutions to ensure they fall within the range
• Small sample consumption-use 2-5 µL of sample (compared to 75 µL for triplicate wells with other methods)
• Fast, easy workflow-2 hours from sample to answer, with no wash steps
• No proprietary instrument to purchase-runs on any real-time PCR instrument
ProQuantum immunoassays utilize a matched pair of target-specific antibodies, each conjugated to a DNA oligonucleotide. During antibody-analyte binding, the two DNA oligos are brought into close proximity, which allows for ligation of the two strands and subsequent creation of a template strand for amplification. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology (Figure 1).
The assay workflow is fast and easy-2 steps in 2 hours. There are a total of 7 components in each kit (Figure 2). First, mix the antibody-conjugates, dilute the sample, and create the standard curve in a working plate. Then, using a multi-channel pipette, add the antibody-conjugates and sample (or standard) into the wells of a PCR plate and incubate for 1 hour. Combine the master mix and ligase and add to the wells of the PCR plate, then run the plate on any qPCR instrument. After the run is complete, import the results file into the ProQuantum software (cloud-based version at https://apps.thermofisher.com/apps/proquantum). Using this software, the data can be analyzed easily to obtain protein concentration values. The software allows you to set up standard curves, design plate layouts, apply a 5PL weighted algorithm, and obtain robust statistical group-wise comparisons.
靶标信息
TGF beta-1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its prodomain. Activation of TGF-b1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF-beta, an aminoterminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP.