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Human IL-1 alpha ProQuantum Immunoassay Kit
- 类型：ProQuantum
- 品牌：Thermo Fisher
- 种属：Human应用：ELISA
规格: 96Tests
货号: A35592
价格: ¥7059.00
优惠: ¥4588.00
规格: 5×96Tests
货号: A355925
价格: ¥31739.00
优惠: ¥20630.00
| 检测灵敏度 | <0.05 pg/mL |
| 检测范围 | 0.064-5,000 pg/mL |
| 样本类型/体积 | {Serum=2-5 µL, Supernatant=2-5 µL, Plasma=2-5 µL} |
| 手工操作时间 | 30 min |
| 总时间 | 2 hr |
| 均相（无需洗涤） | Yes |
| 检测仪器 | qPCR Instruments |
| 产品规格 | 96 Tests |
| 产品成分 |
Assay Dilution Buffer Antibody-Conjugate A Antibody-Conjugate B Antibody-Conjugate Dilution Buffer Standard Master Mix Ligase |
| 运输条件 | Dry ice |
| 储存温度 | -20°C |
| 蛋白名称 | IL-1 alpha |
| 物种（Tested） |
Human |
| 试剂盒类型 | Proximity-based Immunoassay |
| 标签或染料 | FAM™ |
The ProQuantum Human IL-1 alpha Immunoassay Kit is designed to provide highly sensitive quantitative measurements of human IL-1 alpha protein in small sample volumes. Utilizing a proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive Cloud-based software for analytics enables sample-to-answer in just 2 hours.
• High sensitivity-detect low levels of protein with greater sensitivity than traditional methods
• Broad dynamic range-≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range
• Small sample consumption-use 2-5 µL of sample (compared to 75 µL for triplicate wells with other methods)
• Fast, easy workflow-2 hours from sample to answer, with no wash steps
• No proprietary instrument to purchase-runs on any real-time PCR instrument
ProQuantum immunoassays utilize a matched pair of target-specific antibodies, each conjugated to a DNA oligonucleotide. During antibody-analyte binding, the two DNA oligos are brought into close proximity, which allows for ligation of the two strands and subsequent creation of a template strand for amplification. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology (figure below).
靶标信息
IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.