检测灵敏度	1.56 ng/mL
测定范围	1.56-100 ng/mL
检测样本类型/体积	{Plasma=10 µL, Serum=10 µL, Supernatant=10 µL}
手工操作时间	1 hr 45 min
总时间	24 hr 30 min
均相（无需洗涤）	No
仪器设备	Absorbance Microplate Reader
产品规格	10 x 96 tests
产品成分	PCapture Antibody: Pre-titrated, purified antibody Detection Antibody: Detection Antibody: Pre-titrated, HRP-conjugated antibody Standard: Recombinant protein for generating standard curve and calibrating samples 10X Coating Buffer: Buffer for plating the Capture Antibody 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme Substrate: 1X TMB Solution Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
运输条件	Wet ice
储存温度	2-8℃
蛋白名称	Mouse IgG
Target sub-specificity	Total
物种	Mouse
检测试剂盒格式	Uncoated  ELISA Kit with Plates
检测抗体结合物	HRP
标签或染料	HRP

 

The Mouse Total IgG Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse Total IgG. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.

 

原理方法

ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

 

靶标信息  

 

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody's species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.