检测灵敏度	4 pg/mL
检测范围	4-250 pg/mL
样本类型/体积	{Plasma=100 µL, Serum=100 µL, Supernatant=100 µL}
手工操作时间	1 hr 45 min
总时间	24 hr 30 min
均相（无需洗涤）	No
检测仪器	Absorbance Microplate Reader
产品规格	10 x 96 Tests
产品成分	Capture Antibody: Pre-titrated, purified antibody Detection Antibody: Pre-titrated, biotin-conjugated antibody Standard: Recombinant protein for generating standard curve and calibrating samples 10X Coating Buffer: Buffer for plating the Capture Antibody 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme Enzyme: Pre-titrated Avidin-HRP Substrate: 1X TMB Solution Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
运输条件	Wet ice
储存温度	2-8°C
蛋白名称	IL-2
物种（Tested）	Human
试剂盒类型	Uncoated ELISA Kit
检测抗体结合物	Biotin
标签或染料	HRP

 

The Human Interleukin-2 (IL-2) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of Humanmouse IL-2. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.

 

原理方法

ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

 

靶标信息  

 

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.