检测灵敏度	35.3 pg/mL
测定范围	78.1-5,000 pg/mL
检测样本类型/体积	{Plasma=25 µL, Serum=25 µL, Supernatant=25 µL}
手工操作时间	1 hr 20 min
总时间	3 hr 30 min
均相（无需洗涤）	No
批间差异	7.6%
批内差异	4.6%
仪器设备	Absorbance Microplate Reader
产品规格	96 tests
产品成分	Pre-coated 96 well plate Standard Sample Diluent Assay Buffer concentrate HRP-conjugated Detection Antibody Wash Buffer Chromogen Stop Solution Adhesive Plate Covers
运输条件	Wet ice
储存温度	2-8℃
蛋白名称	TIM-3
物种	Human
检测试剂盒格式	Sandwich ELISA Kit
检测抗体结合物	Biotin
标签或染料	HRP

 

The Human T-cell Immunoglobulin and Mucin-domain containing-3 (Hu TIM-3) ELISA quantitates Hu TIM-3 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TIM-3.

 

原理方法  

 

The Human TIM-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

 

严格验证  

 

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

 

靶标信息  

 

TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin, mucin-dmain containing-3) is a 281 amino acid long, Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. TIM3 consists of an IgV-like domain, a mucin-like domain in the extracellular region, and a conserved Tyrosine phosphorylation motif in the cytoplasmic region. TIM3 is involved in macrophage activation and induction of autoimmune diseases. Further, TIM3 down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance. Pathological significance of TIM3 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease, and also enhances the severity of experimental autoimmune encephalomyelitis in mice.