检测灵敏度	0.21 ng/mL
测定范围	0.31-20.0 ng/mL
检测样本类型/体积	{ Plasma=10 µL, Serum=10 µL, Supernatant=10 µL, Urine=10 µL}
手工操作时间	15 min
总时间	3 hr 10 min
均相（无需洗涤）	No
批间差异	10%
批内差异	7.7%
仪器设备	Absorbance Microplate Reader
产品规格	128 tests
产品成分	Pre-coated 96 well Microplate Detection Antibody Anti-IgG-HRP Sample Diluent, lyophilized Standard (8-well strips x 4) Wash Buffer Concentrate Substrate Solution Sample Diluent Stop Solution Lysis Buffer 10x Adhesive Plate Covers
运输条件	Wet ice
储存温度	-20℃
蛋白名称	CD25
Target sub-specificity	Soluble
物种	Human
检测试剂盒格式	Instant ELISA Kit
检测抗体结合物	HRP
标签或染料	HRP

 

The Human Soluble CD25/Interleukin 2 Receptor(Hu sCD25/IL-2R) ELISA quantitates Hu sCD25/IL-2R in human serum, plasma, urine or cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sCD25/IL-2R.

 

原理方法

The Human sCD25/IL-2R solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

 

严格验证

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

 

靶标信息  

 

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.