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Human PD-1 ELISA Kit
- 类型：ELISA试剂盒
- 品牌：Thermo Fisher
- 种属：Human应用：ELISA
规格: 96Tests
货号: BMS2214
价格: ¥6594.00
优惠: ¥4286.00
| 检测灵敏度 | 1.14 pg/mL |
| 检测范围 | 2.34-150 pg/mL |
| 样本类型/体积 | {Plasma=50 µL, Serum=50 µL, Supernatant=50 µL} |
| 手工操作时间 | 1 hr 20 min |
| 总时间 | 3 hr 10 min |
| 均相（无需洗涤） | No |
| 批间差异 | 6.4% |
| 批内差异 | 3.2% |
| 检测仪器 | Absorbance Microplate Reader |
| 产品规格 | 96 Tests |
| 产品成分 |
Pre-coated 96 well plate Standard Sample Diluent Assay Buffer concentrate Biotinylated Detection Antibody SAV-HRP Wash Buffer Chromogen Stop Solution Adhesive Plate Covers |
| 运输条件 | Wet ice |
| 储存温度 | 2-8°C |
| 蛋白名称 | PD-1 (CD279) |
| 物种（Tested） |
Human |
| 试剂盒类型 | Sandwich ELISA Kit |
| 检测抗体结合物 | Biotin |
| 标签或染料 |
HRP |
The Human Programmed T cell Death 1 (Hu PD-1) ELISA quantitates Hu PD-1 in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant H PD-1.
原理方法
The Human PD-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.