检测灵敏度	15.6 ng/mL
检测范围	15.6-1,000 ng/mL
样本类型/体积	{Plasma=10 µL, Serum=10 µL, Supernatant=10 µL}
手工操作时间	1 hr 45 min
总时间	24 hr 30 min
均相（无需洗涤）	No
检测仪器	Absorbance Microplate Reader
产品规格	10 x 96 Tests
产品成分	Capture Antibody: Pre-titrated, purified antibody Detection Antibody: Pre-titrated, HRP-conjugated antibody Standard: Human IgM for generating standard curve and calibrating samples 10X Coating Buffer: Buffer for plating the Capture Antibody 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme Substrate: 1X TMB Solution Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
运输条件	Wet ice
储存温度	2-8°C
蛋白名称	Human IgM
物种（Tested）	Human
试剂盒类型	Uncoated ELISA Kit
检测抗体结合物	HRP
标签或染料	HRP

 

The Human Immunoglobulin M (IgM) Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IgM. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.

 

原理方法

ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

 

靶标信息  

 

IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.