检测灵敏度	1 ng/mL
检测范围	15.6-1,000 ng/mL
样本类型/体积	{Plasma=10 µL, Serum=10 µL, Supernatant=10 µL}
手工操作时间	1 hr 20 min
总时间	1 hr 30 min
批间差异	3.2%
批内差异	5.1%
均相（无需洗涤）	No
检测仪器	Absorbance Microplate Reader
产品规格	96 Tests
产品成分	Pre-coated 96 well plate Standard Sample Diluent Assay Buffer concentrate HRP-conjugated Detection Antibody Wash Buffer Chromogen Stop Solution Adhesive Plate Covers
运输条件	Wet ice
储存温度	2-8°C
蛋白名称	Human IgM
物种（Tested）	Human
试剂盒类型	Sandwich ELISA Kit
检测抗体结合物	HRP
标签或染料	HRP

 

The Human Immunoglobulin M (Hu IgM) ELISA quantitates Hu IgM in human serum, plasma or cell culture supernatant. The assay will exclusively recognize both natural and recombinant Hu IgM.

 

原理方法

The Human IgM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

 

严格验证

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

 

靶标信息  

 

IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.