检测灵敏度	1.25 ng/mL
检测范围	1.25-80 ng/mL
样本类型/体积	{Plasma=10 µL, Serum=10 µL, Supernatant=10 µL}
手工操作时间	1 hr 45 min
总时间	24 hr 30 min
均相（无需洗涤）	No
检测仪器	Absorbance Microplate Reader
产品规格	2 x 96 Tests
产品成分	Capture Antibody: Pre-titrated, purified antibody Detection Antibody: Pre-titrated, HRP-conjugated antibody Standard: Human IgG4 protein for generating standard curve and calibrating samples 10X Coating Buffer: Buffer for plating the Capture Antibody 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme Substrate: 1X TMB Solution Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
运输条件	Wet ice
储存温度	2-8°C
蛋白名称	Human IgG4
物种（Tested）	Human
试剂盒类型	Uncoated ELISA Kit with Plates
检测抗体结合物	HRP
标签或染料	HRP

 

The Human IgG4 Uncoated ELISA Kit contains pre-matched antibody pair, platess and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IgG4. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.

 

原理方法

ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

 

靶标信息  

 

Human IgG4, also known as human immunoglobulin G4, is a subclass of human IgG antibodies that is produced by the immune system in response to antigens. It is the least abundant subclass of IgG in the blood and plays a minor role in defense against pathogens. IgG4 works by binding to specific antigens and promoting their clearance from the body through various mechanisms, including phagocytosis and complement activation. IgG4 is also involved in other immune responses, such as immune tolerance and suppression of inflammation. Human IgG4 can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious and autoimmune diseases.