检测灵敏度	0.24 ng/mL
测定范围	1.6-100 ng/mL
检测样本类型/体积	{Plasma=10 µL, Serum=10 µL}
手工操作时间	1 hr 20 min
总时间	1 hr 30 min
均相（无需洗涤）	No
批间差异	2.6%
批内差异	5.3%
仪器设备	Absorbance Microplate Reader
产品规格	96 tests
产品成分	Pre-coated 96 well plate Standard Sample Diluent Assay Buffer concentrate HRP-conjugated Detection Antibody Wash Buffer Chromogen Stop Solution Adhesive Plate Covers
运输条件	Wet ice
储存温度	2-8℃
蛋白名称	Human IgG
物种	Human
检测试剂盒格式	Sandwich ELISA Kit
检测抗体结合物	HRP
标签或染料	HRP

 

The Human Total Immunoglobulin G (Hu IgG) ELISA quantitates Hu IgG in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu IgG.

 

原理方法

The Human IgG solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

 

严格验证

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

 

靶标信息  

 

Human IgG, also known as human immunoglobulin G, is an antibody that is produced by the immune system in response to antigens. It is the most abundant immunoglobulin in the blood and plays a key role in defense against pathogens. IgG works by binding to specific antigens and promoting their clearance from the body through various mechanisms, including phagocytosis and complement activation. IgG is also involved in other immune responses, such as antibody-dependent cellular cytotoxicity and neutralization of toxins. Human IgG can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious and autoimmune diseases.