检测灵敏度	7.8 ng/mL
测定范围	7.8-500 ng/mL
检测样本类型/体积	{Plasma=10 µL, Serum=10 µL，Supernatant=10 µL}
手工操作时间	1 hr 45 min
总时间	24 hr 30 min
均相（无需洗涤）	No
仪器设备	Absorbance Microplate Reader
产品规格	10×96 tests
产品成分	Capture Antibody: Pre-titrated, purified antibody Detection Antibody: Pre-titrated, HRP-conjugated antibody Standard: Recombinant protein for generating standard curve and calibrating samples 10X Coating Buffer: Buffer for plating the Capture Antibody 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme Substrate: 1X TMB Solution Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
运输条件	Wet ice
储存温度	2-8℃
蛋白名称	Human IgE
物种	Human
检测试剂盒格式	Uncoated ELISA Kit with Plates
检测抗体结合物	Biotin
标签或染料	HRP

 

The Human Immunoglobulin E (Hu IgE) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IgE. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.

 

原理方法

ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

 

靶标信息  

 

IgE is one of the five classes of immunoglobulins found in mammals, characterized by its monomeric structure consisting of two heavy (epsilon) chains with four Ig-like constant domains and two light chains. Although IgE comprises only a small portion of the total immunoglobulin in serum, it plays a crucial role in type I hypersensitivity reactions, such as hay fever, asthma, hives, and anaphylactic shock. IgE is produced by terminally differentiated plasma B cells and basophils. It binds to Fc epsilon receptors on mast cells and basophils, leading to the release of histamine and other molecules that promote allergic responses. Crosslinking of IgE bound to these receptors induces degranulation of mast cells and basophils, resulting in allergic manifestations. In addition to its role in allergic responses, IgE is functionally important for immunity against parasites, highlighting its dual role in both protective and pathological immune processes.