检测灵敏度	0.5 ng/mL
测定范围	7.8-500 ng/mL
检测样本类型/体积	{Plasma=10 µL, Serum=10 µL}
手工操作时间	1 hr 20 min
总时间	1 hr 30 min
均相（无需洗涤）	No
批间差异	5.2%
批内差异	6.6%
仪器设备	Absorbance Microplate Reader
产品规格	96 tests
产品成分	Pre-coated 96 well plate Standard Sample Diluent Assay Buffer concentrate HRP-conjugated Detection Antibody Wash Buffer Chromogen Stop Solution Adhesive Plate Covers
运输条件	Wet ice
储存温度	2-8℃
蛋白名称	Human IgE
物种	Human
检测试剂盒格式	Sandwich ELISA Kit
检测抗体结合物	HRP
标签或染料	HRP

 

The Human Immunoglobulin E (Hu IgE) ELISA quantitates Hu IgE in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu IgE.

 

原理方法

The Human IgE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

 

严格验证

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

 

靶标信息  

 

IgE is one of the five classes of immunoglobulins found in mammals, characterized by its monomeric structure consisting of two heavy (epsilon) chains with four Ig-like constant domains and two light chains. Although IgE comprises only a small portion of the total immunoglobulin in serum, it plays a crucial role in type I hypersensitivity reactions, such as hay fever, asthma, hives, and anaphylactic shock. IgE is produced by terminally differentiated plasma B cells and basophils. It binds to Fc epsilon receptors on mast cells and basophils, leading to the release of histamine and other molecules that promote allergic responses. Crosslinking of IgE bound to these receptors induces degranulation of mast cells and basophils, resulting in allergic manifestations. In addition to its role in allergic responses, IgE is functionally important for immunity against parasites, highlighting its dual role in both protective and pathological immune processes.