检测灵敏度	0.13 ng/mL
测定范围	0.31-20.0 ng/mL
检测样本类型/体积	{Plasma=50 µL, Serum=50 µL, Supernatant=50 µL}
手工操作时间	15 min
总时间	3 hr 10 min
均相（无需洗涤）	No
批间差异	9.8%
批内差异	8.2%
仪器设备	Absorbance Microplate Reader
产品规格	128 tests
产品成分	Pre-coated 96 well Microplate Biotinylated Detection Antibody SAV-HRP Sample Diluent, lyophilized Standard (8-well strips x 4) Wash Buffer Concentrate Substrate Solution Sample Diluent Stop Solution Lysis Buffer 10x Adhesive Plate Covers
运输条件	Wet ice
储存温度	-20℃
蛋白名称	BAFF (BLyS)
物种	Human
检测试剂盒格式	Instant ELISA Kit
检测抗体结合物	Biotin
标签或染料	HRP

 

The Human B cell Activating Factor belonging to the TNF Family (Hu BAFF) ELISA quantitates Hu BAFF in human serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu BAFF.

 

原理方法

The Human BAFF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

 

严格验证

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

 

靶标信息  

 

BAFF is a membrane bound cytokine expressed by B cell lineage cells. The soluble form of the cytokine binds to the receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, and important for the proliferation and differentiation of B cells. BAFF is also expressed by T lymphocytes and has been shown to promote T cell activation and survival. Isoforms representing alternatively spliced, and glycosylated variants have been identified.