Maxima H Minus cDNA 合成预混液以便利的单管预混液形式提供所有 cDNA 合成反应组分。其经过优化，可实现高效的 cDNA 合成，适用于两步法定量 RT-PCR (RT-qPCR) 应用。

特点包括：
•单管预混液有助于减少移液步骤并提高 RT-qPCR 结果的一致性
•可在较宽的 RNA 起始量和基因靶标范围内提高 RT 效率
•高热稳定性，以允许在 50 至 65°C 的温度范围内进行 RT 反应

预混液含有 Maxima H Minus 反转录酶 (RT) 和 RiboLock RNase 抑制剂。Maxima H Minus RT 是一种通过体外进化从 M-MuLV RT 衍生而来的高级酶。该酶具有以下特点：高热稳定性、耐用性、持续合成能力，cDNA 合成速率有所提高，无 RNase H 活性。重组 RiboLock RNase 抑制剂可在高达 55°C 下有效防止 RNases A、B 和 C 引起的 RNA 模板降解。预混液还含有反应缓冲液、dNTP、寡聚 (dT)18 和随机六聚体引物。还提供了一单管无 RT 对照品混合物。

Maxima H Minus cDNA 合成预混液能够在高温 (50–65°C) 下进行可重现 cDNA 合成。合成反应通常在 15–30 分钟内完成。

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qPCR黄金组合：

PowerUp™ SYBR™ Green 预混液

MicroAmp™ 光学 96 孔反应板

MicroAmp™ 光学粘性封板膜

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有关反应组分的其他信息
•无 RT 对照品混合物包含 Maxima H Minus cDNA 合成预混液中除 RT 外的所有组分。无 RT 对照品反应中存在 PCR 产物表明反应被 DNA 污染。为进一步提高基因组 DNA 消除效率，强烈推荐将模板 RNA 与 dsDNase（货号 EN0771）共同孵育。
• 无核酸酶水用于样品 DNA 的反应构建和稀释。经适当的质量检测确认，不含脱氧核糖核酸外切酶、核糖核酸酶以及磷酸酶。

 

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Maxima H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipeting steps, and is optimized for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima H Minus cDNA Synthesis Master Mix is optimized for reproducible cDNA synthesis at elevated temperatures (50–65°C) within 30 minutes.

The master mix contains Maxima H Minus Reverse Transcriptase (RT) and RiboLock RNase Inhibitor. Maxima H Minus RT is an advanced enzyme derived from M-MuLV RT by in vitro evolution. The enzyme features high thermostability, robustness, processivity, increased cDNA synthesis rate, and lack of RNase H activity. The recombinant RiboLock RNase Inhibitor effectively protects RNA template from degradation by RNases A, B, and C at temperatures up to 55°C. The master mix also contains reaction buffer, dNTPs, oligo (dT)18, and random hexamer primers. A separate tube of No-RT control mix is provided for convenient RT negative control.

The included double-strand specific DNase (dsDNase) allows removal of genomic DNA from RNA samples in 2 minutes without affecting the quality or quantity of RNA. This dsDNase is also available separately (Cat. No. EN0771).

Additional information about reaction components
• The No RT Control mix contains all components in the Maxima H Minus cDNA Synthesis Master Mix except Maxima H Minus RT. The presence of a PCR product in the No RT Control reaction indicates that the reaction is contaminated with DNA. To further enhance genomic DNA elimination efficiency, template RNA incubation with the included dsDNase is strongly recommended.
• The dsDNase is used for rapid and safe removal of contaminating genomic DNA from RNA samples. dsDNase is easily inactivated by moderate heat treatment (55°C). It allows for a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure.
• Nuclease-free water is provided for reaction set-up and dilution of sample DNA. The absence of exodeoxyribonucleases, ribonucleases, and phosphatases has been confirmed by appropriate quality tests.

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优点：

-One-tube master mix for reduced pipetting steps and enhanced consistency in RT-qPCR results
- Increased RT efficiency across a wide range of input RNA amounts and gene targets
- High thermostability to allow RT reactions at 50 to 65°C temperature range
- Integrated step of genomic DNA removal from RNA samples

 

组成成分：

▪ 1 x 200 µL Maxima H Minus cDNA Synthesis Master Mix ▪ 1 x 200 µL Maxima H Minus cDNA Synthesis Master Mix No RT Control ▪ 1 x 50 µL dsDNase ▪ 1 x 100 µL 10X dsDNase buffer ▪1 x 1.25 mL nuclease-free water Store at -5 to -30°C.

相关产品：

▪ DNA 片段标准品 ▪ DNA ladder ▪ DNA 聚合酶 ▪ PCR ▪ DNA纯化 ▪ RNA 纯化