描述

Thermo Scientific GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The kit includes a DNase l for the enzymatic removal of genomic DNA. RNA can be isolated from whole blood in less than 30 minutes (including cell lysis and DNase l treatment).

Highlights

• Versatile—compatible with fresh and frozen blood, buffy coat, as well as blood collected in PAXgene™and Tempus™ Tubes
• Convenient—specially optimized Lysis Buffer is suitable for RNA stabilization up to 48 h
• Efficient—up to 4 µg of total RNA from 500 µL of healthy human blood
• Pure—isolates intact total RNA free of any RT-qPCR inhibitors

Applications

Isolated total RNA can be used in:
• RT-PCR
• RT-qPCR
• Northern Blotting

Includes

• Lysis Buffer (R2841)
• Wash Buffer 1 (conc.)(R2851)
• Wash Buffer 2 (conc.)(R2861)
• DNase l (lyophilized)
• DNase I Reconstitution Buffer
• 2x DNase I Buffer
• Manganese Chloride Solution
• Water, nuclease-free
• GeneJET RNA Purification Columns pre-assembled with Collection Tubes
• Collection Tubes, 2 mL
• Collection Tubes, 1.5 mL

Related Products
Lysis Buffer for GeneJET Blood RNA Kit
Wash Buffer 1 for GeneJET Blood RNA Kit (concentrated)
Wash Buffer 2 for GeneJET Blood RNA Kit (concentrated)

Superior yields of isolated mRNA

Total RNA was purified with the Thermo Scientific Kit and other vendors' kits using 250 µL of blood as starting material.

All eluates were brought to the same volume.

RNA was analysed by RT-qPCR using Thermo Scientific Verso 1-Step RT-qPCR SYBR Green Kit (AB-4104/A).