描述

Thermo Scientific GeneJET Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from wide variety of plant species and tissue types.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis. Purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting and other RNA-based analysis. RNA yields vary between different species, tissues, and age of tissue sample. See Table 1 for typical total RNA yields from various sources.

Highlights

• Fast—procedure completed in 20 min following cell lysis
• Convinient—protocol does not include clogging-prone lysate filtration step
• High yield—up to 65 µg of high purity and high integrity RNA from 50 mg plant sample
• Compatible with wide variety of samples (leaves, roots, sprouts and other parts of various plants)

Applications

• Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay

RNA purified from tomato leaves using different kits

1 µL (2% of eluate) of RNA isolated using the GeneJET Plant RNA Purification Mini Kit and the kits from other vendors were fractionated on 1% agarose gel.

M – RiboRuler High Range RNA Ladder (#SM1821)

1 – Thermo Scientific

2-5 – various vendors

High integrity RNA isolated from difficult to process samples using GeneJET Plant RNA Purification Mini Kit

1 µL of total RNA isolated from 50 mg of various plants and plant parts analyzed using Agilent 2100 Bioanalyzer™.

1 – Corn roots

2 – Rape roots

3 – Sunflower stalk

4 – Rape stalk

5 – Cucumber fruit

6 – Tomato fruit

7 – Sunflower seeds

8 – Rape seeds

9 – Corn seeds

Overview of purification procedure