优点：

- Efficient—high yields of high molecular weight genomic DNA
- Fast—20 minute procedure following cell lysis
- Convenient—spin columns are capped and assembled with collection tubes
- Pure—DNA pure from enzyme inhibitors, RNA and proteins. Ready to use for all demanding applications and validated in real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests
- Compatible—suitable for wide variety of samples like fresh or frozen anticoagulated mammalian blood (treated with EDTA, Citrate or Heparine), aged blood, buffy coat, bone marrow, and body fluids

数据：

图1.Accurate evaluation of genomic DNA yields
Genomic DNA was purified from human blood using the GeneJET Whole Blood Genomic DNA Purification Mini Kit and kits from other vendors. DNA concentration was estimated using Nanodrop spectrophotometer (Thermo Scientific) and 200 pg used as template in qPCR with primers against single copy gene. Matching yields obtained by qPCR based absolute quantification and spectrophotometrical evaluation indicate absence of reaction inhibitors and contaminating RNA.

图2.Genomic DNA purified from various sources using the GeneJET Whole Blood Genomic DNA Purification Mini Kit
200 ng of isolated genomic DNA from each source was fractionated by agarose gel (0.5%) electrophoresis.
M – Lambda DNA/HindIII Marker (#SM0101)
1 – human whole blood
2 – human buffy coat
3 – human bone marrow
4 – avian whole blood. 200 ng of isolated genomic DNA from each source was fractionated by agarose gel (0.5%) electrophoresis
M – Lambda DNA/HindIII Marker (#SM0101)
1 – human whole blood
2 – human buffy coat
3 – human bone marrow
4 – avian whole blood

图3.纯化流程

组成成分：

▪ Proteinase K Solution

▪ Lysis Solution

▪ Wash Buffer WB I (concentrated)

▪ Wash Buffer II (concentrated)

▪ Elution Buffer (10 mM Tris-HCl, pH 9.0, 0.1 mM EDTA)

▪ GeneJET Genomic DNA Purification Columns pre-assembled with Collection Tubes

▪ Collection Tubes (2 mL)

相关产品：

▪ DNA 片段标准品

▪ DNA ladder

▪ DNA 聚合酶

▪ PCR

▪ DNA纯化

▪ RNA纯化