灵敏度

 8 pg/mL

The Mouse Tumor Necrosis Factor Alpha (TNF-α) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse TNF-α. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.

小鼠肿瘤坏死因子α（TNF-α）未包被的ELISA试剂盒包含预先匹配的抗体对，板和试剂，用于进行定量酶联免疫吸附测定（ELISA），以检测和定量小鼠TNF-α的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应，需单独购买。

Principle of the method 方法原理

ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。

Target information 靶标信息

Tumor Necrosis Factor is a polypeptide cytokine produced by monocytes and macrophages. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNF-alpha circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting. TNF-alpha also inhibits lipoprotein lipase activity resulting in cachexia, a physical wasting condition. Activation of B-cells by the Epstein Barr virus can be inhibited by TNF-alpha. Due to its varied actions throughout the immune system, TNF-alpha may play a role in the pathogenesis of many disease states.

肿瘤坏死因子是单核细胞和巨噬细胞产生的多肽细胞因子。 它充当免疫反应的多能调节剂，并进一步充当强效热原。 TNF-α在全身循环，对刺激（感染因子或组织损伤）作出反应，激活中性粒细胞，改变血管内皮细胞的特性，调节其他组织的代谢活性，并通过诱导局部凝血而表现出杀肿瘤活性。 TNF-α还抑制脂蛋白脂肪酶的活性，导致恶病质，一种物理上的浪费状态。 爱泼斯坦巴尔病毒对B细胞的激活可以被TNF-α抑制。 由于其在整个免疫系统中的不同作用，TNF-α可能在许多疾病状态的发病机理中起作用。

TNF-alpha production is mediated by the action of lymphokines and endotoxins on the macrophage. Purified monocytes produce TNF-alpha within four hours of stimulation by recombinant IL-2 and there is some in vitro evidence to suggest that TNF-alpha is expressed at high levels and with prolonged kinetics in T cells stimulated by both CD2 and CD28. Secretion of TNF-alpha is enhanced by gamma interferon. TNF then induces or enhances the specific production of Class I MHC antigen, GM-CSF, and IL-1.

TNF-α的产生是由巨噬细胞上淋巴因子和内毒素的作用介导的。 纯化的单核细胞在重组IL-2刺激的四个小时内产生TNF-α，并且有一些体外证据表明TNF-α在被CD2和CD28刺激的T细胞中高水平表达，并且动力学延长。 γ-干扰素可增强TNF-α的分泌。 然后，TNF诱导或增强I类MHC抗原，GM-CSF和IL-1的特异性产生。

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.