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Mouse TNF alpha Uncoated ELISA Kit
- 类型：ELISA试剂盒
- 品牌：Thermo Fisher
- 种属：Mouse应用：ELISA
规格: 10×96Tests
货号: 88-7324-88
价格: ¥6745.00
优惠: ¥4384.00
规格: 20×96TTests
货号: 88-7324-77
价格: ¥9179.00
优惠: ¥5966.00
| 检测灵敏度 | 15.6 pg/mL |
| 检测范围 | 15.6-1,000 pg/mL |
| 样本类型/体积 | {Lavage Fluid=100 µL, Plasma=100 µL, Serum=100 µL, Supernatant=100 µL, Tissue Homogenate=100 µL} |
| 手工操作时间 | 1 hr 45 min |
| 总时间 | 24 hr 30 min |
| 均相（无需洗涤） | No |
| 检测仪器 | Absorbance Microplate Reader |
| 产品规格 | 10 x 96 Tests |
| 产品成分 |
Capture Antibody: Pre-titrated, purified antibody Detection Antibody: Pre-titrated, biotin-conjugated antibody Standard: Recombinant cytokine for generating standard curve and calibrating samples ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20). Assay Diluent: 5X concentrated Detection enzyme: Pre-titrated Avidin-HRP Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| 运输条件 | Wet ice |
| 储存温度 | 2-8°C |
| 蛋白名称 | TNF alpha |
| 物种（Tested） |
Mouse |
| 试剂盒类型 | Uncoated ELISA Kit |
| 检测抗体结合物 | Biotin |
| 标签或染料 | HRP |
The Mouse Tumor Necrosis Factor Alpha (TNF-α) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse TNF-α. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
靶标信息
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.