NK MACS® Medium, human|细胞生物学,细胞培养

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NK MACS® Medium, human

类型：培养基

品牌：Miltenyi

规格: 500 mL

货号: 130-114-429

价格: ¥2255.00

数量:

NK MACS培养基用于培养和扩增人NK细胞的优化细胞培养基。它不含动物成分，但含有稳定的谷氨酰胺和酚红。NK MACS培养基被开发用于人类NK细胞的培养，活化和扩增。扩增的NK细胞具有完全的功能，可用于任何下游分析，例如:杀伤性测定。起始细胞可以是分离的人NK细胞或PBMC。从PBMC开始扩增时，非目的细胞（T细胞，NKT细胞）的生长和仅有极少的扩增。

组成成分：

- 500 mL NK MACS® Basal Medium, human

- 5 mL NK MACS® Supplement (100×), human

数据：

Figure 1
High expansion of NK cells in NK MACS Medium. NK cell fold expansion from PBMCs (n=3) using 5% AB serum and 500 IU/mL of IL-2 and 140 U/mL IL-15 during 14 days showed high expansion rates for both, NK MACS Medium (research use) and NK MACS GMP Medium (Phenol Red).

Figure 2
Expanded NK cells are fully functional and can be used in downstream assays, e.g. cytotoxicity assays. NK cells from PBMCs were expanded for 14 days in NK MACS Medium (research use) and NK MACS GMP Medium (Phenol Red) and were analyzed via flow cytometry for cytotoxicity against cell line K-562 at different effector-to-target (E:T) ratios.

Figure 3
Superior expansion of NK cells in NK MACS Medium compared to standard culture methods

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