The MitoProbe TMRM Assay Kit contains tetramethylrhodamine methyl ester (TMRM) for the detection of mitochondrial membrane potential state and CCCP for the induction of mitochondrial membrane depolarization, if desired.

• Study mitochondrial membrane potential in live cells
• Quick, easy protocol

Determining the state of mitochondrial health can be achieved by detecting changes in the mitochondrial membrane potential. In a healthy cell with active mitochondria, TMRM is readily sequestered, thereby emitting a red-orange fluorescent signal. When apoptosis is induced or a treatment like CCCP is applied, the mitochondrial membrane is depolarized and the TMRM signal diminishes.

Jurkat cells, a human T-lymphocyte celline, were treated with DMS0 (control or 500 nl staurosporine for 2 hours. Cels were subsequentlystained with MitoProbe TMRl for 30 min at 37'c, followed by a wash and additional stain with Annexin V Pacific Blue. Data was acquired on anAttune NxT flow cytometer using a 561 laser and 585/16 nm emission filter for MitoProbe TMRM and 405 laser and 440/50 nm emission filterfor Annexin V Pacific Blue. Staurosporine induced apoptosis, resulting in a mixed population of cells containing a population of healthyMitoProbe TMRiM-positive cells as well as a population of apoptotic, Annexin V Pacific Blue-positive, MitoProbe TMRM-low cells.