应用	建议稀释比
对照试剂 (Ctrl)	Assay-Dependent
流式细胞分析 (Flow)	Assay-Dependent

 

产品详细信息

 

Description: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin.

 

Applications Reported: PE-Cy7 Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis.

 

Applications Tested: Rat IgG2b K Isotype Control PE-Cyanine7 has been tested by flow cytometric analysis of mouse splenocytes. It should be used at the same concentration as the experimental antibody.

 

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

 

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

 

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

 

Filtration: 0.2 µm post-manufacturing filtered.

 

靶标信息

 

Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies. IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization. Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.