应用

建议稀释比

流式细胞分析 (Flow)

1-10 µg/mL

免疫细胞化学 (ICC/IF)

4 µg/mL

产品详细信息

To minimize cross-reactivity, these goat anti-rat IgG whole antibodies have been cross-adsorbed against mouse IgG, mouse serum, and human serum prior to conjugation. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins.

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.

Product will be shipped at Room Temperature.

靶标信息

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

The abundance of pectin associated with the plasmodesmatal pit fields of kiwifruit cells. Pectin, a component of the cell wall matrix and the main constituent of the middle lamella that forms between daughter cell walls, was tagged with an anti-pectin monoclonal antibody, JIM 5. The primary antibody was detected and visualized with Alexa Fluor® 488 goat anti-rat IgG (Product # A-11006). The primary antibody was a gift from Dr. Paul Knox, University of Leeds, U.K. Image contributed by Paul Sutherland, The Horticulture and Food Research Institute of New Zealand, Ltd., Mt. Albert Research Centre.

Immunofluorescence analysis of Polyoma Virus Medium T (green) in PyMT+ mammary tumor cells and mammary gland lymph node. Cells were stained with a Polyoma Virus Medium T Monoclonal Antibody (Product # MA1-46061) at a dilution of 1:500 overnight at 4C, and then incubated with secondary goat anti-rat IgG - Alexa Fluor 488 antibody (Product # A-11006) at a dilution of 1:500 for 1 hour. Data courtesy of Antibody Data Exchange Program.