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Chicken anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647
- 类型:荧光二抗
- 品牌:Thermo Fisher
- 种属:Rat应用:WB,ICC/IF,IHC(P)抗体亚型:IgY偶联物:Alexa Fluor 647/AF647宿主:Chicken浓度:2 mg/mL
规格: 1mg
货号: A21472
价格: ¥3647.00
应用 |
建议稀释比 |
免疫组化(石蜡) (IHC (P)) |
1-10 µg/mL |
免疫细胞化学 (ICC/IF) |
1:1,000-1:2,000 |
免疫印迹 (WB) |
1:5,000-1:10,000 |
产品详细信息
Chicken anti-rat antibodies react with IgG heavy chains and all classes of immunoglobulin light chains from rat. Chicken secondary antibodies have gained popularity because they demonstrate a lower level of nonspecific binding. Chicken antibodies lack a classic “Fc” domain and will not bind to protein A or protein G, nor will they bind to mammalian IgG Fc receptors. Fluorescence of this long-wavelength Alexa Fluor dye is not visible by looking through a conventional fluorescence microscope.
靶标信息
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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