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Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor 647
- 类型:荧光二抗
- 品牌:Thermo Fisher
- 种属:Chicken应用:WB,IHC,ICC/IF抗体亚型:IgG偶联物:Alexa Fluor 647/AF647宿主:Goat浓度:2 mg/mL
规格: 1mg
货号: A21449
价格: ¥3359.00
优惠: ¥2355.00
应用 |
建议稀释比 |
免疫组化 (IHC) |
1-10 µg/mL |
免疫细胞化学 (ICC/IF) |
1-10 µg/mL |
免疫印迹 (WB) |
1:5,000-1:10,000 |
产品详细信息
Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 647 dye is a near-infrared-fluorescent dye with excitation ideally suited to the 647 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.
靶标信息
Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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