Overview

The Central Memory CD4+ T Cell Isolation Kit was developed for the isolation of central memory CD4+ T cells from human PBMCs in a two-step procedure.

Central memory CD4+ T cells are isolated based on the expression of CD197 (CCR7) and the absence of CD45RA on these cells.

Detailed separation procedure

For the first separation, non-target cells are labeled with a cocktail of biotin-conjugated antibodies against CD8, CD14, CD15, CD16, CD19, CD25, CD36, CD45RA, CD56, CD123, TCRγ/δ, and CD235a (Glycophorin A) and Anti-Biotin MicroBeads to deplete non-CD4+ T cells, naive CD4+ T cells and non T cells. For the second separation, the flow-through fraction of the first separation is labeled with CD197 (CCR7)-PE and Anti-PE MicroBeads for positive selection of central memory CD4+ T cells.

 

相关产品：

 

 ▪ MS分选柱（货号#130-042-201）

 ▪ LS分选柱（货号#130-042-401）

 ▪ 磁力架（货号#130-042-303）

 ▪ MS分选磁极（货号#130-042-102）

 ▪ ImunoSep buffer（货号#604025/604050）

 

数据：

图 1.使用CD4 +中央记忆T细胞分离试剂盒，LS和MS柱，MidiMACS TM分离器和MiniMACS TM分离器从人PBMC分离CD4 +中枢记忆T细胞。 用CD4-APC和CD45RA-FITC染色细胞。 在分离过程中，细胞已经用CD197（CCR7）-PE标记。