Overview

The Effector Memory CD4+ T Cell Isolation Kit was developed for the isolation of untouched memory T helper cells from human peripheral blood.

Detailed separation procedure

For magnetic depletion of non-target cells, PBMCs are incubated with a cocktail of biotinylated antibodies against CD45RA, CD8, CD14, CD15, CD16, CD19, CD34, CD36, CD56, CD123, Anti-TCRγ/δ, and CD235a (glycophorin A) as well as APC-conjugated CD197 (CCR7) antibodies. In a second step, all antibody-labeled cells are labeled with MicroBead Cocktail for depletion. The CD4+ Effector Memory T Cell Isolation Kit provides a fast and efficient way to isolate untouched CD4+ effector memory T cells.

 

Downstream applications

CD4+ effector memory T cells isolated with the CD4+ Effector Memory T Cell Isolation Kit can be used for functional studies, for studies on signal transduction, or for analysis of cytokine expression.

 

相关产品：

 

 ▪ MS分选柱（货号#130-042-201）

 ▪ LS分选柱（货号#130-042-401）

 ▪ 磁力架（货号#130-042-303）

 ▪ MS分选磁极（货号#130-042-102）

 ▪ ImunoSep buffer（货号#604025/604050）

 

数据：

图 1.使用CD4 +效应记忆T细胞分离试剂盒，LD柱和MidiMACS TM分离器从人PBMC分离未经标记的CD4 +效应记忆T细胞。 用CD4-FITC和CD45RO-PE染色细胞。 在分离过程中用CD197（CCR7）-APC标记细胞。