Overview

 

CD62L MicroBeads were developed for the positive selection or depletion of human CD62L+ cells from PBMCs, body fluids (e.g. bronchial lavage), or single-cell suspensions from tissue (e.g. lymphoid or tumor tissue).

 

 

Detailed product information

 

Background information

 

The CD62L antigen is a 74 kDa glycoprotein and is a member of the selectin family of cell surface molecules, also referred to as L-selectin, LECAM-1, or LAM-1. CD62L binds a series of glycoproteins including CD34, GlyCAM-1, and MAdCAM-1. CD62L is important for homing of naive lymphocytes via the high endothelial venules to peripheral lymph nodes and Peyer's patches. The CD62L antigen also contributes to the recruitment of leukocytes from the blood to areas of inflammation. Most hematopoietic cells, including most peripheral blood B cells, T cells, monocytes, dendritic cells, granulocytes, and some myeloid cells from bone marrow and thymocytes, express CD62L. CD62L is continuously endoproteolytically cleaved from the cell surface of CD62L-expressing neutrophils and lymphocytes (shedding). Proteolysis is accelerated, e.g., after antigen activation of T cells.

CD62L+ T cells can also be selected from pre-enriched CD4+ or CD8+ T cells using the CD4+ T Cell Isolation Kit or CD8+ T Cell Isolation Kit. CD4+CD62L+ central memory T cells can be isolated from CD4+ memory T cells, which were pre-enriched with the Memory CD4+ T Cell Isolation Kit.

 

 

Columns

 

For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.