您的位置: 首页 > 产品分类 > 抗体和ELISA >二抗 >荧光二抗 >Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488/驴抗兔 IgG (H+L)高交叉吸附荧光二抗,Alexa Fluor Plus 488

Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488/驴抗兔 IgG (H+L)高交叉吸附荧光二抗,Alexa Fluor Plus 488

货号:A32790

规格:1 mg

价格:3726

产品类型:荧光二抗

品牌:Thermo Fisher

物种:

宿主:

抗体亚型:IgG

荧光染料:其它

产品介绍

36个不同种属、不同荧光的Alexa Fluor plus荧光二抗

点击表格中的货号,可跳转到相应的产品页。


Invitrogen Alexa Fluor Plus Secondary   Antibody products


Alexa Fluor Plus 488

Alexa   Fluor Plus 555

Alexa Fluor   Plus 594

Alexa   Fluor Plus 647

Alexa   Fluor Plus 680

Alexa   Fluor Plus 800

Goat anti-Rabbit

A32731 (launched   2016)

A32732   (launched 2016)

A32740

A32733   (launched 2016)

A32734 (launched   2016)

A32735   (launched 2016)

Goat anti-Mouse

A32723 (launched   2016)

A32727 (launched   2016)

A32742

A32728 (launched   2016)

A32729   (launched 2016)

A32730   (launched 2016)

Goat anti-Chicken

A32931

A32932

A32759

A32933

A32934

A32935

Donkey anti-Goat

A32814

A32816

A32758

A32849

A32860

A32930

Donkey anti-Rabbit

A32790

A32794

A32754

A32795

A32802

A32808

Donkey anti-Mouse

A32766

A32773

A32744

A32787

A32788

A32789

产品优势:

1.     更多荧光选择(从480800),更多种属选择;

2.     更好的光稳定性,更高信噪比,更高灵敏度,经过交叉吸附检测;

3.     发布1年多已经有多达99篇高分文章引用;

4.     适用于ICC/IF/WB等实验应用。

抗体类型:

荧光二抗

同型对照:

 IgG

免疫原性:

Gamma Immunoglobins Heavy and Light chains

用法:

1-10µg/mL(ICC);1-10µg/mL(IF);0.1-0.4µg/mL(WB)

To minimize cross-reactivity, the donkey anti-mouse IgG whole antibodies have been cross-adsorbed against IgG from bovine, goat, chicken, guinea pig, hamster, horse, sheep, mouse, rat, and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.

Specificity: This antibody binds to heavy chains on mouse IgG and light chains on all rabbit immunoglobulins. This antibody does not bind non-immunoglobulin rabbit serum proteins or IgG from bovine, goat, chicken, guinea pig, hamster, horse, sheep, mouse, rat, or human.

Background/Target Information

Obtain superior images with the new Invitrogen™ Alexa Fluor™ Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor™ Plus secondary antibodies provide brighter signal while having lower cross-reactivity compared to leading Alexa Fluor secondary antibodies, providing you with higher sensitivity and better signal-to-noise for your critical experiments.

Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry and are pre-adsorbed to reduce background and cross-reactivity so you can generate stunning images. Alexa Fluor Plus secondary antibodies provide up to 4.2 fold higher signal-to-background in imaging applications and up to 5.8 fold higher signal-to-background in fluorescent western applications compared to Alexa Fluor secondary conjugates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.


数据

1.jpg                                  

Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A32790) in IF

Immunofluorescent analysis of tubulin in U2OS cells. The cells were fixed with 4% formaldehyde for 20 mins, permeabilized with 0.5% Triton X-100 in PBS for 20 mins, washed 3X in PBS and blocked with 3% BSA in PBS for 30 mins at RT. Cells were stained with a tubulin antibody at a dilution of 1:1000 in 3% BSA in PBS for 1 hr at RT, washed 3X in PBS and then incubated with Invitrogen Alexa Fluor Plus 488 donkey anti-rabbit IgG secondary antibody (Product # A32790) prepared in 3% BSA in PBS at a dilution of 1:1000 for 1 hr at RT in the presence of NucBlue Live ReadyProbes Reagent (Product # R37605). The image contains overlay of tubulin (red) and nuclei (blue). Images were taken on an EVOS FL Auto 2 Imaging System (Product # AMAFD2000) with an Olympus 20X Super Apochromat objective (Product # AMEP4734) at 20X magnification. Actin was stained using Alexa Fluor Plus Phalloidin (Product # A30105).

A32790-DAR488-40x-U2OS-1_20180829011558.jpg

Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A32790) in IF

Immunofluorescent analysis of tubulin in U2OS cells. The cells were fixed with 4% formaldehyde for 20 mins, permeabilized with 0.5% Triton X-100 in PBS for 20 mins, washed 3X in PBS and blocked with 3% BSA in PBS for 30 mins at RT. Cells were stained with a tubulin antibody at a dilution of 1:1000 in 3% BSA in PBS for 1 hr at RT, washed 3X in PBS and then incubated with Invitrogen Alexa Fluor Plus 488 donkey anti-rabbit IgG secondary antibody (Product # A32790) prepared in 3% BSA in PBS at a dilution of 1:1000 for 1 hr at RT in the presence of NucBlue Live ReadyProbes Reagent (Product # R37605). The image contains overlay of tubulin (red) and nuclei (blue). Images were taken on an EVOS FL Auto 2 Imaging System (Product # AMAFD2000) with an Olympus 40X Super Apochromat objective (Product # AMEP4754) at 40X magnification. Actin was stained using Alexa Fluor Plus Phalloidin (Product # A30105).


3.jpg

Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A32790) in IF

Immunofluorescent analysis of tubulin in U2OS cells. The cells were fixed with 4% formaldehyde for 20 mins, permeabilized with 0.5% Triton X-100 in PBS for 20 mins, washed 3X in PBS and blocked with 3% BSA in PBS for 30 mins at RT. Cells were stained with a tubulin antibody at a dilution of 1:1000 in 3% BSA in PBS for 1 hr at RT, washed 3X in PBS and then incubated with Invitrogen Alexa Fluor Plus 488 donkey anti-rabbit IgG secondary antibody (Product # A32790) prepared in 3% BSA in PBS at a dilution of 1:1000 for 1 hr at RT in the presence of NucBlue Live ReadyProbes Reagent (Product # R37605). The image contains overlay of tubulin (red) and nuclei (blue). Images were taken on an EVOS FL Auto 2 Imaging System (Product # AMAFD2000) with an Olympus 40X Super Apochromat objective (Product # AMEP4754) at 40X magnificatio

                       

4.jpg

Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A32790) in IF

Immunofluorescent analysis of tubulin in U2OS cells. The cells were fixed with 4% formaldehyde for 20 mins, permeabilized with 0.5% Triton X-100 in PBS for 20 mins, washed 3X in PBS and blocked with 3% BSA in PBS for 30 mins at RT. Cells were stained with a tubulin antibody at a dilution of 1:1000 in 3% BSA in PBS for 1 hr at RT, washed 3X in PBS and then incubated with Invitrogen Alexa Fluor Plus 488 donkey anti-rabbit IgG secondary antibody (Product # A32790) prepared in 3% BSA in PBS at a dilution of 1:1000 for 1 hr at RT in the presence of NucBlue Live ReadyProbes Reagent (Product # R37605). The image contains overlay of tubulin (red) and nuclei (blue). Images were taken on an EVOS FL Auto 2 Imaging System (Product # AMAFD2000) with an Olympus 20X Super Apochromat objective (Product # AMEP4734) at 20X magnification

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荧光二抗

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技术参数

产品优点 1. 灵敏度更高,信噪比更好,光稳定性更强;
2. 亮度高,耐淬灭,对PH值不敏感;
3. 能高灵敏的检测低丰度蛋白。

产品应用 ICC;IF;WB

实验方法和说明书下载

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