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CD15 Monoclonal Antibody (MMA), Alexa Fluor™ 514
- 类型：一抗
- 品牌：Thermo Fisher
- 种属：Human应用：IHC(P)抗体亚型：IgM偶联物：Alexa Fluor 514/AF514宿主：Mouse克隆号：MMA浓度：0.2 mg/mL
规格: 500 µL
货号: 753-0158-94
价格: ¥5190.00
促销: ¥3633.00
应用 |
建议稀释比 |
|
免疫组化（石蜡） (IHC (P)) |
1 µg/mL |
|
产品详细信息
Description: The MMA monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative.
Applications Reported: This MMA antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of paraffin-embedded tissue.
Applications Tested: This MMA antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue with high pH antigen retrieval at 1 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
靶标信息
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.